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Gibson assembly is a DNA assembly method which allows for the joining of multiple DNA fragments in a single, isothermal reaction. It was invented in 2009 by Daniel Gibson while he was at the J. Craig Venter Institute (JCVI). == Process == The entire Gibson assembly reaction requires a small number of components with very few manipulations.〔 The method can simultaneously combine numerous (>10) DNA fragments based on sequence identity. It requires that the DNA fragments contain ~20-40 base pair overlap with adjacent DNA fragments. These DNA fragments are mixed with a cocktail of three enzymes, along with other buffer components. The three required enzyme activities are: exonuclease, DNA polymerase, and DNA ligase. * The exonuclease chews back DNA from the 5' end. The resulting single-stranded regions on adjacent DNA fragments can anneal. * The DNA polymerase incorporates nucleotides to fill in any gaps. * The DNA ligase covalently joins the DNA of adjacent segments, thereby removing any nicks in the DNA. The entire mixture is incubated at 50 °C for up to one hour. The resulting product is different DNA fragments joined into one. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Gibson assembly」の詳細全文を読む スポンサード リンク
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